cdna gearray human common cytokine microarray  (SuperArray Bioscience Corporation)

Journal:

Article Title: Endothelial Cells Promote Human Immunodeficiency Virus Replication in Nondividing Memory T Cells via Nef-, Vpr-, and T-Cell Receptor-Dependent Activation of NFAT

doi: 10.1128/JVI.79.17.11194-11204.2005

Figure Lengend Snippet: Changes in gene expression induced by HIV infection. FACS analyses after 9 days of EC-T-cell coculture of (A) uninfected CD4+ cells, (B) wild-type HIV-infected T cells without selection, and (C) wild-type HIV-infected T cells following depletion of CD4high cells. (D) FACS histogram of replicate uninfected cultures without sorting (top) and after sorting (bottom) for the CFSEhigh population. (E) Human Common Cytokine Superarray Analyses of RNAs isolated from CD4low cells from wild-type virus-infected EC-T-cell cocultures compared with unactivated CD4+ T cells from the same donor. Cytokine expression levels were normalized to GAPDH. Depicted are genes that displayed a >1.5-fold change in expression in CD4low cells (presumably infected) versus CFSEhigh cells (from uninfected cultures). The data in panels A to E are from one of three experiments with similar results.

Article Snippet: Cytokine expression was assessed using a cDNA GEArray Human Common Cytokine Microarray (Superarray, Frederick, MD).

Techniques: Expressing, Infection, Selection, Isolation

Journal:

Article Title: Endothelial Cells Promote Human Immunodeficiency Virus Replication in Nondividing Memory T Cells via Nef-, Vpr-, and T-Cell Receptor-Dependent Activation of NFAT

doi: 10.1128/JVI.79.17.11194-11204.2005

Figure Lengend Snippet: TCRs and not cytokine signals provide minimal activation necessary for HIV replication. (A) Replication of wild-type HIV in EC-T-cell cocultures 9 days postinfection treated with the indicated blocking antibodies (dark bars) or isotype controls (light bars) as assessed by p24 measured in the supernatant by ELISA. (B) IL-2 measured in the supernatant of infected T cells after 1 day of coculture with ECs in the presence of various doses of PHA-L or vehicle alone. (C) Cell-free virus measured in the supernatant of infected EC-T-cell cocultures on day 7 postinfection as assessed by p24 measured in the supernatant by ELISA. (D and E) FACS analyses of CD4+ T cells cocultured with untreated ECs in the presence of (D) 0 μg/ml and (E) 0.063 μg/ml of PHA-L. The boxes identify the CFSEhigh p24high cells. Note the expansion of CFSEhigh p24high cells in the wild-type-infected cultures in the presence of 0.063 μg/ml of PHA-L. The data in panels A to E are from one of three experiments with similar results. The error bars indicate standard deviations.

Article Snippet: Cytokine expression was assessed using a cDNA GEArray Human Common Cytokine Microarray (Superarray, Frederick, MD).

Techniques: Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Infection